Cloned (Comment) | Organism |
---|---|
gene MGAT5, overexpression in CHO cells and Lec4 cells from Cricetulus griseus | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of cell line Lec4A lacking N-acetylglucosaminyltransferase V activity and protein in the Golgi apparatus | Cricetulus griseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Cricetulus griseus | 5794 | - |
Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | P97259 | gene MGAT5 | - |
Rattus norvegicus | Q08834 | gene MGAT5 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | - |
Cricetulus griseus | - |
CHO-Lec4 cell | - |
Cricetulus griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + octyl 6-O-[2-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-alpha-D-mannopyranosyl]-beta-D-glucopyranoside | a synthetic trisaccharide acceptor | Cricetulus griseus | UDP + ? | - |
? | |
UDP-N-acetyl-D-glucosamine + octyl 6-O-[2-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-alpha-D-mannopyranosyl]-beta-D-glucopyranoside | a synthetic trisaccharide acceptor | Rattus norvegicus | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glc-NAcT-V | - |
Cricetulus griseus |
Glc-NAcT-V | - |
Rattus norvegicus |
GlcNAcT-V | - |
Cricetulus griseus |
GlcNAcT-V | - |
Rattus norvegicus |
Mgat5 | - |
Cricetulus griseus |
Mgat5 | - |
Rattus norvegicus |
N-acetylglucosaminyltransferase V | - |
Cricetulus griseus |
N-acetylglucosaminyltransferase V | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cricetulus griseus |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Cricetulus griseus |
6.5 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | absense of the enzyme is due to a base insertion at nucleotide 822 of the Magt5 gene that shifts the open reading frame. A 155 amino acid truncated GlcNAcT-V (instead of a full length 740 amino acid enzyme) may be synthesized, which consists of the cytosolic and transmembrane domains and a short piece of the stem region, the truncated enzyme is degenerated. Lack of the enzyme protein causes a reduction in Golgi volume density in Lec4A cells, this can be reversed by stable transfection of Lec4A cells with a cDNA encoding Mgat5. No effect on Golgi volume density is observed in CHO Lec1 cells that contain enzymatically active GlcNAcT-V, but cannot synthesize beta1,6-branched glycans due to an inactive GlcNAcT-I in their Golgi apparatus. The structure of the Golgi apparatus in cells stably transfected and therefore overexpressing different glycosyltransferases appears normal | Cricetulus griseus |
malfunction | absense of the enzyme is due to a base insertion at nucleotide 822 of the Magt5 gene that shifts the open reading frame. A 155 amino acid truncated GlcNAcT-V (instead of a full length 740 amino acid enzyme) may be synthesized, which consists of the cytosolic and transmembrane domains and a short piece of the stem region, the truncated enzyme is degenerated. Lack of the enzyme protein causes a reduction in Golgi volume density in Lec4A cells, this can be reversed by stable transfection of Lec4A cells with a cDNA encoding Mgat5. No effect on Golgi volume density is observed in CHO Lec1 cells that contain enzymatically active GlcNAcT-V, but cannot synthesize beta1,6-branched glycans due to an inactive GlcNAcT-I in their Golgi apparatus. The structure of the Golgi apparatus in cells stably transfected and therefore overexpressing different glycosyltransferases appears normal | Rattus norvegicus |